FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_E1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_E1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12120852
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC363010.29949214791171447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA236170.194846038875815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT225070.18568826679840658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1215400.096.970231
AGCAGTG1358500.086.648312
GCAGTGG1590550.073.940593
CAGTGGT1695300.069.315934
AGTGGTA1723050.068.344535
TGGTATC1777500.066.163527
GTGGTAT1790050.065.74856
GGTATCA1794550.065.55898
GTATCAA1832550.064.18049
CAACGCA1825600.036.32748412-13
ATCAACG1837750.036.15760410-11
GCAGAGT1841950.035.79051216-17
AGAGTAC1840250.035.726318-19
ACGCAGA1871450.035.37026614-15
TACATGG1862500.034.69943222-23
AGTACAT1933450.033.89690420-21
CATGGGG547250.033.20987724-25
TCAACGC1813450.032.32948712-13
CATGGGA1234450.031.82101224-25
AACGCAG1854100.031.60463514-15
Produced by FastQC (version 0.11.5)