FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_E1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_E1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13711066
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC418260.3050528675159174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA271060.19769432952915553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT261040.19038636383195878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1398750.097.0714951
AGCAGTG1562250.086.7616042
GCAGTGG1826150.074.134313
CAGTGGT1939100.069.7762454
AGTGGTA1971500.068.695335
TGGTATC2024200.066.643097
GTGGTAT2043700.066.196846
GGTATCA2044300.066.0116968
GTATCAA2083450.064.75389
CAACGCA2084500.036.52269412-13
ATCAACG2099600.036.30726610-11
GCAGAGT2103850.036.01148616-17
AGAGTAC2105300.035.91596618-19
ACGCAGA2133300.035.64828514-15
TACATGG2123650.034.9396322-23
AGTACAT2210350.034.10219620-21
CATGGGG622900.033.93524-25
TCAACGC2072400.032.5176812-13
CATGGGA1406550.031.99599824-25
CAGAGTA2113650.031.76705418-19
Produced by FastQC (version 0.11.5)