FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_E1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_E1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10884044
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC326670.300136603637398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA217140.19950305235811247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT202520.18607054510253726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1104300.097.056951
AGCAGTG1236450.086.602122
GCAGTGG1452800.073.696153
CAGTGGT1547650.069.081024
AGTGGTA1571050.068.138895
TGGTATC1612450.066.216017
GTGGTAT1627200.065.767676
GGTATCA1628200.065.598758
GTATCAA1662850.064.147979
CAACGCA1653050.036.46587412-13
ATCAACG1665400.036.26217710-11
GCAGAGT1675400.035.8458816-17
AGAGTAC1673100.035.81524318-19
ACGCAGA1697500.035.4698814-15
TACATGG1691350.034.87029622-23
AGTACAT1763750.033.8990520-21
CATGGGG494900.033.4216524-25
TCAACGC1643050.032.45454412-13
CATGGGA1121750.031.94512624-25
AACGCAG1684500.031.62374714-15
Produced by FastQC (version 0.11.5)