FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_514hpf_E1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_514hpf_E1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10668027
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC324440.3041237147225068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA210440.19726234288683372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT200710.18814163106261353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1073700.097.015331
AGCAGTG1199300.086.747722
GCAGTGG1409550.073.77493
CAGTGGT1501350.069.301494
AGTGGTA1527100.068.171345
TGGTATC1568450.066.1355067
GTGGTAT1584500.065.621896
GGTATCA1582100.065.533268
GTATCAA1614700.064.271679
CAACGCA1614500.036.14941812-13
ATCAACG1622350.036.01060510-11
GCAGAGT1626700.035.70095416-17
AGAGTAC1621750.035.6584418-19
ACGCAGA1649850.035.3330714-15
TACATGG1645200.034.65728422-23
AGTACAT1709850.033.7344520-21
CATGGGG485950.033.42276424-25
TCAACGC1601850.032.32651512-13
CATGGGA1089200.031.70460924-25
CAGAGTA1632400.031.55331618-19
Produced by FastQC (version 0.11.5)