FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_16.5hpf_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_16.5hpf_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11608349
Sequences flagged as poor quality0
Sequence length110
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC132540.11417644317895681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT685300.094.5811
AGCAGTG768350.084.115712
GCAGTGG886900.072.806823
CAGTGGT938400.068.797184
TGGTATC974550.066.164127
AGTGGTA982400.065.91375
GGTATCA982350.065.654938
GTGGTAT992600.065.164716
GTATCAA1009450.063.965019
CAACGCA1004050.035.81958412-13
GCAGAGT1008700.035.42907716-17
AGAGTAC1007300.035.4270718-19
ATCAACG1049150.034.3956210-11
TACATGG1025950.034.1598822-23
AGTACAT1070400.033.23719420-21
ACGCAGA1097800.032.75398314-15
CATGGGG324950.032.3365824-25
TCAACGC1016750.031.70248812-13
CAGAGTA1015400.031.56702218-19
CATGGGA667800.031.39194124-25
Produced by FastQC (version 0.11.5)