FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_16.5hpf_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_16.5hpf_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13950022
Sequences flagged as poor quality0
Sequence length110
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC157570.11295322688379988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT823100.094.4475561
AGCAGTG926150.083.816962
GCAGTGG1074650.072.117433
CAGTGGT1138300.068.046194
AGTGGTA1187900.065.251545
TGGTATC1187850.065.1011357
GGTATCA1191700.064.839848
GTGGTAT1207500.064.173166
GTATCAA1226900.063.0146149
CAACGCA1218800.035.63696712-13
GCAGAGT1221450.035.29386516-17
AGAGTAC1218250.035.2846918-19
ATCAACG1271400.034.25756510-11
TACATGG1242900.033.98322322-23
AGTACAT1294100.033.13313720-21
ACGCAGA1324900.032.69883314-15
CATGGGG389000.032.3809124-25
TCAACGC1228700.031.47497712-13
CATGGGA812400.031.2690524-25
CAGAGTA1230350.031.16303318-19
Produced by FastQC (version 0.11.5)