FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_16.5hpf_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_16.5hpf_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14416002
Sequences flagged as poor quality0
Sequence length110
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC163950.11372778666373659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT850950.094.775491
AGCAGTG956950.084.237672
GCAGTGG1106950.072.74413
CAGTGGT1168100.068.848844
TGGTATC1209300.066.316397
GGTATCA1214000.066.068688
AGTGGTA1219500.066.039495
GTGGTAT1228050.065.484596
GTATCAA1244850.064.427859
CAACGCA1237500.036.16945312-13
AGAGTAC1244000.035.72670418-19
GCAGAGT1246650.035.72566216-17
ATCAACG1291900.034.77029410-11
TACATGG1268400.034.34868622-23
AGTACAT1319650.033.58817720-21
ACGCAGA1354700.032.99260314-15
CATGGGG394800.032.49716624-25
TCAACGC1249650.032.06622312-13
CAGAGTA1256400.031.72120518-19
CATGGGA831300.031.7209724-25
Produced by FastQC (version 0.11.5)