FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_WE_16.5hpf_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_WE_16.5hpf_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14654079
Sequences flagged as poor quality0
Sequence length110
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC171020.11670470726955955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT868850.094.6195141
AGCAGTG977450.084.024532
GCAGTGG1132500.072.4033
CAGTGGT1197550.068.432454
AGTGGTA1245400.065.893335
TGGTATC1245700.065.656537
GGTATCA1250200.065.425048
GTGGTAT1267550.064.675896
GTATCAA1282700.063.7878469
CAACGCA1274100.035.9264512-13
GCAGAGT1280550.035.5693216-17
AGAGTAC1284050.035.42204718-19
ATCAACG1334800.034.39334510-11
TACATGG1303250.034.1908922-23
AGTACAT1360250.033.32417720-21
CATGGGG412850.032.9977324-25
ACGCAGA1387100.032.95466214-15
TCAACGC1286700.031.7330512-13
CAGAGTA1290550.031.46379518-19
CATGGGA845100.031.45254324-25
Produced by FastQC (version 0.11.5)