FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_HTO2_16.5hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_HTO2_16.5hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6741546
Sequences flagged as poor quality0
Sequence length110
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG369681954.83636839383726No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1229731.8241068146683268No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA641420.9514434819550293No Hit
ACATGTTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA545020.8084495752161299No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524700.7783081210155653No Hit
ACATGTTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493710.7323394366811411No Hit
ACATGTTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415670.6165796391510197No Hit
ACATGTTACCGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252780.3749585035835994No Hit
ACATGTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236680.35107674115106535No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG113160.16785467309723912No Hit
ACATGTTACCGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79460.1178661393098853No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT77790.11538896270974046No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG74060.1098561071896565No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72530.10758659808892501No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68070.10097090489332862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTG900.0104.0050059
ATCTCAT154.6178608E-4104.0050059
GTATTCG201.1125596E-5104.0050059
AGATACT154.6178608E-4104.0050059
ACTTGAA201.1125596E-5104.0050059
TTTCGCA450.0104.0050059
CAGCATA154.6178608E-4104.0050059
CCGTGCA900.0104.0050059
ACTTCAA351.6189006E-10104.0050059
CTCTTTG550.0104.0050059
ATGTCAG154.6178608E-4104.0050059
CCGTCGA1800.0104.0050059
CCGTCAC154.6178608E-4104.0050059
TTTCACG1200.0104.0050059
GCATTCG500.0104.0050059
GCATTCC403.6379788E-12104.0050059
ATATCAT252.6955422E-7104.0050059
CACTCAA154.6178608E-4104.0050059
CCTTTAA550.0104.0050059
CTGCCAA154.6178608E-4104.0050059
Produced by FastQC (version 0.11.5)