FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n02_dpErk_HTO1_5-14hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n02_dpErk_HTO1_5-14hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22842481
Sequences flagged as poor quality0
Sequence length110
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG766051433.536260794087994No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9779824.2814175920732955No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4931822.1590561900872327No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2596961.1368992711430952No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2248490.9843457897590021No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG876200.3835835520668705No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG576850.2525338644256725No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT575470.2519297268978794No Hit
AGGACCATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA519820.22756722441839833No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG474620.20777953147908934No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG450440.19719399131819348No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC385880.168930861757092No Hit
AGGACCATCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA382200.1673198283496438No Hit
AGGACCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA379430.16610717548588527No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT359630.15743911530450655No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGG347590.1521682342649207No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG322650.14124998068292144No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA314500.13768206702240443No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT306500.13417982048447363No Hit
AAGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300600.13159691366274967No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGG273290.11964111954388844No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAA263760.11546906835557837No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA259420.1135690996087509No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG256410.11225137934885446No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT255780.11197557743399239No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA244860.10719501090971685No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG243880.10676598570882033No Hit
AGGACCATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240700.10537384270999284No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA235530.10311051588485506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCAG351.6189006E-10104.006439
CTTCATC154.6177526E-4104.0064249
CTTCACT154.6177526E-4104.0064249
GCGTTCG154.6177526E-4104.0064249
TTTCACG2450.0104.0064249
CTTCTTA201.112541E-5104.0064249
CAGCTCA201.112541E-5104.0064249
ACTCCTG154.6177526E-4104.0064249
TCCTGCA154.6180745E-4104.004618
TCTTCCC154.6180745E-4104.004618
TCCCTAA600.0104.004618
TCCTAGA154.6180745E-4104.004618
TCCTAAT154.6180745E-4104.004618
TCCACCC154.6180745E-4104.004618
TCACTCA154.6180745E-4104.004618
TCCATTC1500.0104.00468
GTTTCGC900.0104.00468
TCCGACA900.0104.00468
TCCTATA201.1126378E-5104.00468
TCAGCCT450.0104.00468
Produced by FastQC (version 0.11.5)