FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47842289
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG8204531.7149116757352474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA25500.017.316271
GCCCGAA18750.016.3265231
GGGCCAT23750.015.6248721
GGGCTAC18200.015.4283321
CTGGACG19150.015.2379771
GAGGGGG14950.015.2316652
AGGGGGG15350.014.9055273
TCCCGAG29550.014.8879368
GGAGGGG15400.014.8728021
CCCGGAA17350.014.8513721
GAGACCC21800.014.7999881
TGCGACG17800.014.5996411
ATGGGAG18800.014.46736051
GGGACCT16800.014.4199441
GGGACAA24250.014.3491291
CGGAGAA20300.014.0492751
AGCCACG21350.014.0288231
GGGATCC21900.014.0284691
AGGGAAG13200.013.9999893
GCCATGG25400.013.7861691
Produced by FastQC (version 0.11.5)