FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_H1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_H1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10101821
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA20650.017.4740261
GGGCTAC16500.015.93503951
CTGGACG15200.015.7780051
GGGACCT14500.015.174041
AAACCCA17350.015.1543611
GCCCGAA14700.015.1172661
CGAAAAG9350.015.0607353
AATGGAA22300.014.9971211
TCGGGAC16600.014.9775081
TGCGACG18250.014.9495461
GTGCTGG15950.014.8981481
GGGATCC19950.014.8888131
TGCGGAG15150.014.8863783
AAACGCT19500.014.8374861
CTGGCAG21100.014.598721
GAAGCCC23450.014.5900491
AGCCACG19900.014.5392461
AGCGCCA23250.014.5262871
TCGAGAA17650.014.3964338
ATGGGAG15650.014.3401941
Produced by FastQC (version 0.11.5)