FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_H1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_H1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12976321
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA25050.016.9080541
CTGGACG19950.016.3777481
GCCCGAA19450.015.5544141
GGGCTAC21900.015.4720281
TGCGACG22500.015.2550171
GGGACCT18850.015.0573651
GAGACCC24450.014.9832491
GAAGCCC25900.014.8665061
AATGGAA29050.014.8071421
ATGGGAG22300.014.7505061
AAACGCT22100.014.7346561
AACCTGA18850.014.7071941
TGGAACT24750.014.6238351
GTGCTGG20650.014.5972531
AGGGAAG15550.014.5743483
AGCCACG26500.014.4468771
AGTAAAC21050.014.3703598
CCGGGTA22300.014.3558441
GGAGAAC21750.014.3142231
TCGAGAA22400.014.2899948
Produced by FastQC (version 0.11.5)