FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_H1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_H1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11752017
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA24600.016.76961
GCCCGAA16700.016.0072361
CTGGACG17750.015.7420731
GGGCTAC19300.015.6747971
AATGGAA26750.015.54513551
GGGACCT16850.015.4730171
GAAGCCC24450.015.342711
TGCGACG20800.015.2848441
AAACGCT19350.014.9520691
GGGATCC22500.014.8144871
ATGGGAG19000.014.7064091
AGCGCCA28450.014.6935871
GGGCCAT20000.014.5761351
CCGGGTA19050.014.4945671
AAACCCA19750.014.48214151
AGCCACG23100.014.4773191
GAGACCC21200.014.3737621
AGTAAAC18450.014.3685238
GGAATGG21600.014.3622311
TCGGGAC17700.014.2327481