FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_G1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_G1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11562152
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA16000.017.1206021
CCGGACA23150.016.3948611
GGGACCT18450.015.5626581
TGCGACG20500.015.4016661
GAAGCCC24750.015.33499051
GGGCTAC20900.015.3174461
CTGGACG18200.015.1719691
TGCGGAG16550.015.1561773
TCGAGAA19100.015.0888758
AGCGCCA26400.015.0432931
AAACGAA21650.014.8376321
GAGACCC22650.014.7653941
AGCCACG22950.014.7641241
GTGCTGG18800.014.68775751
GGCACGT20500.014.5430361
ATGGGAG18600.014.3725211
TGGAACT20150.014.30427551
CCGGGTA19500.014.2733391
AAACCCA18150.014.18335151
TCGGGAC18100.014.1617521
Produced by FastQC (version 0.11.5)