FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_G1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_G1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11220335
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA23950.017.1330361
CTGGACG18100.015.8632811
GGGCTAC18850.015.5822781
GCCCGAA16800.015.5192281
GGCACGT19600.015.2666541
TTGACCC20700.015.0399761
GAGACCC21400.014.9592651
AATGGAA24500.014.9523411
ATGGGAG18750.014.7266341
AAACGAA18950.014.5712071
AGCGCCA25050.014.4922981
TCGAGAA18750.014.4905898
TGGAACT19450.014.4228681
AGTAAAC19000.014.4157148
GGGATCC20950.014.3879061
GTTGAAC20800.014.3858861
GGGACCT15650.014.3399261
AAACGCT20300.014.3066831
AGGCTGC21400.014.2909811
ATCGGCG13400.014.2848431