FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_G1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_G1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10967995
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA22650.016.9020941
CTGGACG16550.016.7506661
GGGACCT15700.016.1160141
GCCCGAA16200.016.0260491
AGGGAAG9950.015.9213093
GAAGCCC21600.015.8393061
TGCGACG19950.015.2744771
GAGACCC21200.015.1522281
GGGCTAC18000.014.9734921
TCGGGAC16350.014.8697611
AGTAAAC18250.014.7068898
GACCGTG19450.014.7056171
GGAGAAC19400.014.4599891
AATGGAA25200.014.4496361
TATATCC21700.014.3975331
GGGATCC21550.014.3956521
GAACGAG14250.014.3594393
TGCGGAG14300.014.3092313
CCCTGAT20050.014.2655491
AGCGCCA24300.014.1699251
Produced by FastQC (version 0.11.5)