FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_G1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_G1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10667248
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA22450.016.9545521
GGGCTAC16950.016.0307481
AAACGCT19250.015.4297741
TGCGACG18350.015.4071961
ATGGGAG18250.015.3710621
GAGACCC22050.015.2166071
GGGCCAT21800.015.1892581
GCCCGAA14950.015.1583741
AGGGAAG13350.015.0804643
GGGATCC20950.014.9128581
AATGGAA23950.014.8821631
AAACGAA18000.014.8511581
GCGCCAC11550.014.7633322
CTGGACG16400.014.7572481
AGCGCCA25800.014.7530881
AGCCACG22450.014.6514771
CCGGGTA17550.014.6051281
AGGCTGC19000.014.5906121
TGGAACT20300.014.52331
GGAGAAC18700.014.4128891
Produced by FastQC (version 0.11.5)