FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_F1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_F1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12351654
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA24850.016.8216381
GCCCGAA16950.016.6142731
GGGCTAC19550.015.8114111
CTGGACG18700.015.6477221
AAACGCT20150.015.2314131
GAAGCCC25400.015.1581221
CTGGCAG24100.015.0628791
ATGGGAG19700.015.0209391
CCCGAAG21850.015.0029241
GAGACCC26250.015.002541
AATGGAA26400.015.0006351
GGGATCC24350.014.8630511
AACGGGA15300.014.7391851
TGCGGAG15450.014.7390253
GTGGAAC13750.014.6402648
TATATCC25700.014.6387521
AAACGAA21000.014.6149041
TTGACCC24100.014.6064281
GGGCCAT24550.014.5179271
GCGGAAA17350.014.392541
Produced by FastQC (version 0.11.5)