FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_F1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_F1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12488764
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA25000.017.1171591
GGGCTAC20400.016.6628931
GCCCGAA18950.015.6158321
CTGGACG18100.015.5590661
GGGACCT19450.015.4406331
GAAGCCC25300.015.04449751
GGGATCC23050.015.0335561
GAACGAG15700.014.7848563
AGTAAAC21000.014.7715428
GGGCCAT24100.014.6980911
GCGACGA9600.014.66795352
AAACGCT22350.014.667661
TGGGCTG21600.014.4639421
CCGGGTA20200.014.4316691
GTGGAGA24100.014.4242121
TGCGACG21100.014.3895991
GAGACCC23450.014.3080041
CATGACG16300.014.2393728
AAACGAA19950.014.2265271
TCGAGAA21950.014.1823418
Produced by FastQC (version 0.11.5)