FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_F1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_F1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11879602
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA25750.017.7725871
GGGCTAC18850.015.9325711
CTGGACG16850.015.9303211
GCCCGAA17250.015.8797921
GGGATCC23600.015.47606851
AGGGAAG12100.015.2744573
AACGGGA13350.015.2449161
GGGACCT17750.015.1225851
ATGGGAG19100.015.0328641
TCGGGAC18650.014.9826771
AAACGAA21700.014.8539671
AAACGCT22450.014.7497511
TTGACCC21250.014.6508251
AGCCACG23350.014.6052631
AATGGAA27000.014.5051031
GGAGAAC21900.014.4169151
TCGAGAA21100.014.388568
TATATCC23350.014.3696951
GCGGAAA15950.014.34621051
GAAGCCC24500.014.323831
Produced by FastQC (version 0.11.5)