FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_F1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_F1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12446909
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA26000.017.5169161
CTGGACG18050.016.8214151
GGGCTAC22100.015.7796591
GCCCGAA17200.015.4780991
GGGACCT19450.015.2712591
GGGCCAT23250.015.1884251
AATGGAA28750.015.0761011
GAGACCC24600.015.0257041
AGCCACG25450.014.7832181
ATGGGAG19800.014.779071
GTGGAAC13650.014.747128
GGCACGT22700.014.7325661
GGGATCC25050.014.7118661
GAAGCCC26100.014.667951
AAACGCT22000.014.6012771
TGCGACG20000.014.4662651
AAACGAA21750.014.4656341
AGGGAAG14150.014.383233
CCGGGTA20400.014.3443921
TGGGCTG23600.014.1707311
Produced by FastQC (version 0.11.5)