FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_E1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_E1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12120852
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGACG17300.016.7875611
CCGGACA23850.016.743571
GGGCTAC19800.016.6680931
GCCCGAA17100.015.6972491
AAACGCT21900.015.47164251
GGGATCC25150.015.3532021
AATGGAA26800.015.0647211
TGCGACG20450.015.0624131
TGGAACT24950.015.0353541
CGGGAGA5150.014.7399144
ATACCGA20400.014.5600681
TCGCCGA13150.014.5551778
GTTGAAC23200.014.5098611
AACGGGA14950.014.4226391
AGTAAAC19700.014.4061358
GGGCCAT24150.014.3946051
AACCTGA17350.014.3931611
TTGACCC23550.014.3876421
TCGAGAA23100.014.3809968
GAGACCC23550.014.3409291