FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_E1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_E1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13711066
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA28750.017.3720131
GCCCGAA17600.016.50151
GGGACCT20550.015.2568611
GGGCTAC21250.015.1684381
CTGGACG21050.014.9467251
GCGACGA9300.014.9046892
AAACCCA24300.014.8490851
TGCGACG22650.014.8137071
GCGGAAA17700.014.7923051
GAGACCC27150.014.78955651
AATGGAA28500.014.7837991
GGCACGT23600.014.7767661
TGGAACT25450.014.6968161
GAACGAG17850.014.6682143
GTGGAGA23350.014.6051821
AGTAAAC22600.014.6017128
GAAGCCC29450.014.5310311
ATGGGAG22500.014.5213181
TGCGGAG19750.014.482543
AGCCACG29700.014.3716751
Produced by FastQC (version 0.11.5)