FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_E1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_E1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10884044
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA22050.017.8113371
GGGCTAC17250.015.6247781
GAGACCC23250.015.5671741
CTGGACG16550.015.3550351
GGCACGT19500.015.3451511
ATGGGAG16650.015.3288861
GAACGAG12650.015.0452853
GCGGAAA14800.015.0150411
TGCGACG17600.015.0015261
GCCCGAA15450.014.9529781
GGGATCC20750.014.9509181
TGGAACT19950.014.7784451
AAACCCA18700.014.7073791
AAACGCT21150.014.6161441
GGAGAAC17600.014.5639811
GAAGCCC21650.014.4818421
AATGGAA24700.014.3860781
CTGGCAG21000.014.353841
TCGGGAC18450.014.2507711
TAACGAC16300.014.2407121
Produced by FastQC (version 0.11.5)