FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_514hpf_E1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_514hpf_E1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10668027
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA14700.017.5866451
CCGGACA19650.016.5155071
CTGGACG17200.016.3096351
TGCGACG17400.015.8692691
GGGCTAC18250.015.7932271
CGAAAAG11750.015.3547423
AGGGAAG12150.015.3019573
GGGACCT15900.015.1523321
GGGATCC20800.015.0734971
ATGGGAG16450.015.0469721
CTGGCAG20900.014.9487371
GGGCCAT19100.014.9175961
GAGACCC23450.014.8712991
GCGACGA7550.014.8624282
GAAGCCC23250.014.8572751
TCGGGAC17450.014.75206851
AATGGAA22900.014.7480761
TGCGGAG14700.014.7429863
AGCCACG20150.014.5770181
GTGCTGG17300.014.4984391
Produced by FastQC (version 0.11.5)