FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_16.5hpf_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_16.5hpf_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11608349
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGA72000.020.0902927
TCCCGAG77050.019.7448
CCCGAGT53400.019.1772589
CCCTCCC52650.018.9725365
CCGGACA33700.018.4110181
AAAGAGT54900.018.3548856
GCCCGAA27750.018.1168141
CAAAGAG58100.018.0638435
GCCATGG44600.017.9566421
AGCCGGA23450.017.9186828
CCTCCCG56600.017.8424636
TACCCGA29400.017.771519
ACCCGAC29500.017.67390410
TAACGAC34800.017.6393871
GCTTTGA56850.017.58772310
ATGTCCC70350.017.5607991
CCCGAGG25400.017.538789
GAAGGGC17350.017.5000046
CACTAAG41300.017.47361
AAGCCTA33900.017.2301037
Produced by FastQC (version 0.11.5)