FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_16.5hpf_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_16.5hpf_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13950022
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGA85800.020.2948067
TCCCGAG94400.019.6923798
CCCGAGT64250.019.1403839
CCCTCCC60750.018.8334435
CCGGACA44400.018.8060151
TAACGAC44200.018.0697561
GTGGGAC31500.018.0535858
GCCCGAA30250.018.0018771
AAAGAGT65750.017.936156
GCTTTGA64700.017.90218510
ATGTCCC80500.017.8478241
ACCCGAC33100.017.8454810
CAAAGAG69100.017.8312845
GCCATGG53700.017.7411421
AGCCGGA26550.017.6493438
GGGCCAT42900.017.6428661
TACCCGA33700.017.5603969
CCCGAGG31100.017.5076059
CACTAAG49100.017.3867441
CCTCCCG68200.017.3079476