FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_16.5hpf_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_16.5hpf_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14416002
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGA88200.020.0544417
TCCCGAG94100.019.5915368
CCCGAGT64850.018.963329
CCCTCCC62100.018.7605535
CCGGACA43950.018.7230471
AAAGAGT67800.018.2051356
CAAAGAG70650.018.0784575
GCCCGAA29750.017.9714471
ATGTCCC81150.017.8131471
GCTTTGA68700.017.7241710
TAACGAC42750.017.6273981
AGCTCAA53800.017.5239621
AGCCGGA27650.017.5041738
GAAGGGC24850.017.4864436
CACTAAG53000.017.4563671
GTGGGAC35650.017.4330338
CCCGAGG31300.017.4309029
GCCATGG54550.017.4040281
AGCCTAG40450.017.3766638
CCTCCCG68900.017.3716246