FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_WE_16.5hpf_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_WE_16.5hpf_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14654079
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGA86050.020.2105167
TCCCGAG95250.019.3552158
CCCGAGT63000.019.1710039
CCCTCCC60650.018.9188985
CCGGACA46150.018.688581
AGCCGGA27550.018.6060128
GCTTTGA71000.018.12623610
AAAGAGT66000.018.0516326
GCCCGAA30550.018.040911
CAAAGAG68850.018.0078685
GTGGGAC37050.017.9917498
TAACGAC44150.017.815881
CACTAAG53450.017.7621291
ATGTCCC80450.017.6809041
TACCCGA38000.017.6575019
ACCCGAC38350.017.49623310
GGGACAA43650.017.3646851
CCTCCCG66750.017.3378986
GCCATGG55000.017.2015551
AGCTCAA53350.017.197431
Produced by FastQC (version 0.11.5)