FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_HTO2_16.5hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_HTO2_16.5hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6741546
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG70980.10528742220256304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAG32900.019.4947743
CGAAACA37800.019.3251023
AGAACCA37150.019.0117343
ACGAAAC31900.019.0024552
AGGGAAG17700.018.708493
ACGAAAA30200.018.50572
GAAAAGA19600.018.354414
CCCGAAG20400.018.2278441
AGAAAAG30650.018.162083
GGGAAGA15050.017.9823884
AGAGGGA14000.017.9158026
ACAGAAA57500.017.8701341
AAACGAA44900.017.7394851
GAAGAGG15650.017.5741544
GAGAAAT47450.017.5280821
CGAAAGA13300.017.3706654
TTATACA62500.017.33566722
GAAACAA32900.017.221074
GAACCAA16550.017.2167244
CATGCGT20950.017.1190283
Produced by FastQC (version 0.11.5)