FastQCFastQC Report
Tue 23 Jul 2019
HVGW3BGXB_n01_dpErk_HTO1_5-14hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGW3BGXB_n01_dpErk_HTO1_5-14hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22842481
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAAAG68750.017.7139173
CGAAAAG56350.017.6878053
AAACGAA116550.017.5375271
AGAACCA73200.017.3283773
ACAGAAA149350.016.868061
GAAAAGA43500.016.4894664
TTATACA138450.016.47740722
CGAAACA75100.016.4505143
CAGAAAA52200.016.311932
ACGAAAA49300.016.2003572
ACGAAAC56300.016.1401122
GCCCGAA26000.015.95155051
GAGAAAT134050.015.847121
TTGACCC41300.015.5826281
AATGGAA72500.015.5077151
CTGGACG41050.015.3827381
GGGCTAC33100.015.1887881
ACAAAAG49400.015.1878783
CCCGAAG36050.015.1665221
AAACCCA69150.015.161271
Produced by FastQC (version 0.11.5)