FastQCFastQC Report
Fri 19 Jul 2019
HVGNWBGXB_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGNWBGXB_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281410717
Sequences flagged as poor quality0
Sequence length99
%GC33

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23942910.8508172771543736No Hit
GTATCAACGCAGAGTGAATGGGAGATCGGAAGAGCGTCGTGTAGGGAAAG9617000.3417424930550886Illumina Single End PCR Primer 1 (100% over 28bp)
GTGAATGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG6680120.23737972992691675Illumina Single End PCR Primer 1 (100% over 41bp)
TATCAACGCAGAGTGAATGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGA5103120.18134064169276112Illumina Single End PCR Primer 1 (100% over 29bp)
CCTATATATTGTATCCTTTACATAAGAATTGCGTATAGATAGATACTATA4311850.15322266493496764No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC4120150.1464105576334536No Hit
GTTTAATAATTCTTATATAATCATCATATACACTATTTTTAGTTCTTAAT3869790.13751395260472613No Hit
CTTTAAAGATGTTTCTTACGCGTTGCGTGTAAAACATCCTCTCATTCAAG3680310.13078073355678205No Hit
ATTATATACTATCATTATCTATAGTTCCAACTTTTCTACGTTTTTCTAGC3327840.11825562421633004No Hit
CTATTTATATAGTGCTTTATATATGTCTCACATAGTTTAATCAATTGTCC3324790.11814724170579474No Hit
ATTATGGACCATGAGGAAGCTAGAAGAAAGCACGTTTCCGGTAAAATCTT3002810.10670560211820221No Hit
GAGTGAATGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2988360.10619211776501036Illumina Single End PCR Primer 1 (100% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5185000.044.2884561
CAACGCA5056450.042.803015
AACGCAG5721550.037.9097186
TCAACGC5791100.037.8210684
ATCAACG6000950.036.392233
CGCAGAG5870100.036.246418
GCAGAGT6238050.033.7617159
ACGCAGA6687050.032.3488967
TATCAAC7011050.031.5936782
AGAGTGA6120650.026.3141910-11
GGTATCA2605350.026.3071561
CTGTGCA550700.025.6077189
GCCCTAC1062150.025.031851
ACTGTGC664650.024.925158
CCTACAC1153900.024.1091843
CAGAGTG5798550.023.1662310-11
TACACGG1152700.022.9921255
CCCTACA1204250.022.875262
ACAGCGT681700.022.7398368
CCTATAT3272000.022.6871571
Produced by FastQC (version 0.11.5)