FastQCFastQC Report
Fri 19 Jul 2019
HVGNWBGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGNWBGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281410717
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG26232165793.21665492931459Illumina Single End PCR Primer 1 (100% over 8bp)
TGCTCTCG111945183.9779998854841057No Hit
TGATCTCG27806840.9881229931978746Illumina PCR Primer Index 5 (100% over 8bp)
CCCTCTCG9398970.33399474263803536TruSeq Adapter, Index 16 (100% over 8bp)
AAATCTCG6269410.22278504766398075RNA PCR Primer, Index 13 (100% over 8bp)
ATATCTCG5802680.20619968073213074Illumina PCR Primer Index 6 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)