FastQCFastQC Report
Fri 19 Jul 2019
HVGNWBGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGNWBGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281410717
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTCACTCTGCGTTGATACCTGTC12292760.4368262918714641No Hit
CCCATTCACCTGTCTCTTATACACATC9435760.3353020844618366No Hit
CCCATTCACTCTGCGTTGATACTGTCT6574850.23363893422722773No Hit
CCCATTCACTCCTGTCTCTTATACACA4501370.15995730539288594No Hit
CCCATTCACTCTGCGTTGATACCCTGT3490590.12403898604899258No Hit
CCCATTCCTGTCTCTTATACACATCTC3466540.12318436330198469No Hit
CCCATTCACTCTGCGTCTGTCTCTTAT3131690.11128538505518254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTAG63826100.021.067975
TTCTAGC63840250.021.0586766
GATCCGT50912400.021.046035
TCTAGCA63849500.021.0386547
TACTAGG27981400.021.0355366
GATTCTA18605450.021.0345134
CTGTCCG37313750.021.033197
TATTCTA15099700.021.0256524
GTACTAG28027500.021.0222435
CTAGCAT63839550.021.0200868
TGATCCG13236650.020.9997884
AATTCTA15840300.020.9944364
TCTGTCC37610550.020.9931766
GGATCCG13823900.020.9911124
CGATCCG11261200.020.9878714
TGTCCGC37394350.020.9754818
GTCAATG30803400.020.9736028
CCGTTAT51006400.020.9704118
ACTAGGT28039650.020.9669657
GTCACTA18235450.020.9547168
Produced by FastQC (version 0.11.5)