FastQCFastQC Report
Sat 22 Feb 2020
HVGK7BGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGK7BGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460539388
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31182140.6770786780130954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT20830800.066.3297961
AGCAGTG21669050.063.5906872
GCAGTGG24554150.056.1162723
AGTGGTA25851150.053.2939725
CAGTGGT27956150.049.265614
GTGGTAT28095450.048.97046
GGTATCA28647650.047.8834848
GTATCAA28811050.047.268059
TGGTATC29447450.046.653917
ACGCAGA27557050.028.6018514-15
CAACGCA27591000.028.53088812-13
ATCAACG28295300.027.9087710-11
AGTACAT28270300.027.89216420-21
GCAGAGT28489800.027.86214316-17
TACATGG28541900.027.51037222-23
AGAGTAC28997000.027.41211318-19
CATGGGG18988100.026.90812324-25
GTACATG27478050.024.84765222-23
TATCAAC28054550.024.34510210-11
CGCAGAG28328400.024.33006916-17
Produced by FastQC (version 0.11.5)