FastQCFastQC Report
Sat 22 Feb 2020
HVGK7BGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGK7BGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460539388
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC6196310813.4544643986021No Hit
CTTATGAG5587093412.131629879179846No Hit
GGCTAGCG5331283611.57617293746002No Hit
ATGACCGC5213431111.320271915591288No Hit
AGACCATA5104617711.083998096597114No Hit
GAGGATCT4934127010.71380022765827No Hit
CAAGTAAA4916070910.67459380911845No Hit
TCTCGTTT456258789.907052293212324No Hit
GGGGGGGG179556343.8988269989189286No Hit
ATGCCCGC17373270.3772374405465619No Hit
AGCCCATA17166400.37274553376529No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)