FastQCFastQC Report
Sat 22 Feb 2020
HVGK7BGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGK7BGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460539388
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG33543390.7283500797981692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTGG1183400.017.0136281
TCTGAGA1084550.016.6944858
AGGGAAG1145050.016.6499883
CTGGACG794650.016.5676821
GCCAGGT1508350.016.4180321
CCCCAGT847650.016.3761435
AAAGTGA1512100.016.2572481
GTGAGAC1019050.016.2325738
CTGATCC1315450.016.1196211
GGCGAGG524150.016.0836034
CTATCCG1323900.016.0150741
TTCCCGA1210250.016.0088797
GATAGAA1517250.015.8793451
AGCCCCA902450.015.7913983
CTGCCTA1066150.015.781291
GGGATCC852600.015.7655321
TGCCGAG1375700.015.6967891
ATGTCCC1324700.015.6706571
GGGACCT704250.015.6242081
TCGAGAC643200.015.5905545
Produced by FastQC (version 0.11.5)