FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n02_plasmid758-B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n02_plasmid758-B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16203337
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG184216311.36903466242787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7735124.773782091923411No Hit
TGGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTA3672082.2662492300197177No Hit
GGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTAT733070.4524191529189327No Hit
CCCAGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG355030.21910918720014277No Hit
CCCCGCAAGCATATTCGGCCAGGATCTCGGACTTGGATCATTAACCTGAG354960.21906598622246765No Hit
CCACGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG308080.19013367431659295No Hit
CCCCGCAAGCATATTCGGACCGGATCTCGGACTTGGATCATTAACCTGAG228030.14073027056093446No Hit
CGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAAT215420.13294792301116737No Hit
CCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATAT196100.12102445317282483No Hit
CCCCGCAAGCATATTCGGCCCGGATCTAGGACTTGGATCATTAACCTGAG190080.11730916909276157No Hit
CCCCGCAAGCATATTCGGCACGGATCTCGGACTTGGATCATTAACCTGAG189420.11690184558896725No Hit
TCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCA189150.11673521324650594No Hit
CTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATC162330.10018306722868259No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)