FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n02_plasmid758-A2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n02_plasmid758-A2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16124978
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG13451378.341946264980951No Hit
TGGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTA2303471.4285104761073162No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2227921.381657699005853No Hit
GGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTAT274920.1704932558667677No Hit
CCCAGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG235370.14596609062040272No Hit
CGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAAT210150.13032575920413658No Hit
CCACGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG209570.12996606879091557No Hit
CCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATAT199590.1237769130599744No Hit
CCCCGCAAGCATATTCGGCCAGGATCTCGGACTTGGATCATTAACCTGAG195180.12104202560772487No Hit
TCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCA171810.10654898257845685No Hit
TGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTT162060.10050246270103438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)