FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n02_plasmid758-A1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n02_plasmid758-A1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8847921
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG6114226.91034650964899No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1240181.401662605260603No Hit
TGGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTA489440.5531694959753822No Hit
CCCAGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG123200.13924174955902072No Hit
CCACGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG118190.1335794024381547No Hit
CCCCGCAAGCATATTCGGCCAGGATCTCGGACTTGGATCATTAACCTGAG114100.12895684760295667No Hit
GGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTAT92980.10508683339283884No Hit
CGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAAT92740.10481558323136023No Hit
CCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATAT88560.10009130958560775No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)