FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8329250
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG940901.1296335204250083No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC356270.4277335894588348TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGCCAATATCTCGTATGC307010.36859261037908575TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGC199540.2395653870396494TruSeq Adapter, Index 6 (98% over 50bp)
GGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTAT160220.19235825554521715No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTAACGCCAATATCTCGTATGC87790.10539964582645496TruSeq Adapter, Index 6 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)