FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n01_plasmid758-B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n01_plasmid758-B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16203337
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG15637989.651086069492969No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5479803.381895963775857TruSeq Adapter, Index 7 (100% over 50bp)
GGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTAT4484622.7677138357364286No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC559250.3451449537832855TruSeq Adapter, Index 7 (98% over 50bp)
CGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAAT275080.16976749912687739No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC267980.16538568567696887TruSeq Adapter, Index 7 (98% over 50bp)
CCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATAT236640.1460439908149784No Hit
CTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATC225200.13898371674921037No Hit
ATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGT219880.13570044244589863No Hit
GACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATG219250.13531163364682225No Hit
TTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTA216520.13362679551749124No Hit
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTTAG211220.13035586435065813No Hit
TCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCA208180.1284797076059086No Hit
TTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGT205400.12676401163538104No Hit
TCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAA197320.12177738449802038No Hit
AGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG187090.11546387018920856No Hit
ATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAA173710.10720631188501478No Hit
CCACGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG172460.10643486585510133No Hit
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTTGATCATTAACCTGAG170260.10507712084245363No Hit
CGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCAT170130.10499689045534262No Hit
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAT168700.10411435619712162No Hit
CCCCGCAAGCATATTCGGCCCTGATCTCGGACTTGGATCATTAACCTGAG167900.10362063073797699No Hit
CCCAGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG164430.10147909655893722No Hit
TGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCG163290.10077553777965613No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)