FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n01_plasmid758-B1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n01_plasmid758-B1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17982089
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC662050836.817235194420405TruSeq Adapter, Index 6 (100% over 50bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC422690.2350616772055794TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC367660.204459003622994TruSeq Adapter, Index 6 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC359750.2000601821067619TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC343990.19129590560918702TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCAATATCTCGTATGC290550.16157744520116657TruSeq Adapter, Index 6 (98% over 50bp)
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG273570.15213471582751037No Hit
GGGAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCTCTGCTCCTTGTG259000.14403220893857213No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAAGC249420.13870468553458945TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCAAGTCACGCCAATATCTCGTATGC246750.13721987473201805TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATGC243720.1355348647201112TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGC235110.13074676696350462TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC234040.13015173042464642TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC232870.12950108299430615TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACGCCAATATCTCGTATGC213700.11884047509719253TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAATATCTCGTATGC209940.11674950557746656TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC204680.11382437268550945TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATTC192050.1068007170913235TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGC186850.10390895073425561TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)