FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n01_plasmid758-A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n01_plasmid758-A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11536113
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3941463.416627420345137TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1007560.873396437777612TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC210110.1821324045629581TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC201310.17450418524853217TruSeq Adapter, Index 5 (98% over 50bp)
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG162890.1412000731962317No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)