FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n01_plasmid758-A2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n01_plasmid758-A2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16124978
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG14141918.770188709714828No Hit
GGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTAT1982941.2297319103319087No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1625101.007815328492231TruSeq Adapter, Index 4 (100% over 50bp)
CGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAAT222050.13770561423401634No Hit
CCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATAT217550.13491491275212902No Hit
CTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATC193250.11984512474993764No Hit
TCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCA179630.1113986015980921No Hit
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTTAG175150.10862030323390208No Hit
TTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTAT174330.10811177540831374No Hit
TTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTA164380.1019412243539185No Hit
TAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATA162250.1006202923191585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)