FastQCFastQC Report
Thu 15 Feb 2024
HVGFWAFX5_n01_plasmid758-A1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVGFWAFX5_n01_plasmid758-A1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8847921
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTGAG6361907.190276676294917No Hit
GGGAAGGGCATGGTATAAAGGAAGCAGTGTATCCGGACTTAATATTTTAT649400.7339577286008769No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC531350.6005365554235849TruSeq Adapter, Index 2 (100% over 50bp)
CGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAAT106260.12009600899465535No Hit
CCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATAT98210.11099782649506024No Hit
CCCCGCAAGCATATTCGGCCCGGATCTCGGACTTGGATCATTAACCTTAG90350.10211438370663571No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)