FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_input_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_input_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40789533
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAT492140.12065350196581069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC102300.047.618344
TATGCCG103000.046.85873446
GTATGCC113150.043.14331445
CACGGCT117350.042.56889331
ATGCCGT115700.041.68528447
ACGTCTG125950.040.64776615
TGCCGTC123200.038.47551748
GCACACG135950.037.83498811
CGTCTGA136500.037.5818616
ACACGTC137650.037.34288413
CACACGT141400.036.5475812
TCTCGTA135250.036.1190341
GCTACAT147550.033.87942535
CATCTCG144950.033.8207139
ATCTCGT149550.032.9190740
CTACATC153750.032.40143636
AGTCACG163550.030.5864928
TCGGAAG177500.029.1807333
ACGGCTA177150.028.60796432
AGCACAC183950.028.37489310
Produced by FastQC (version 0.11.2)