FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_input_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_input_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30513984
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG436680.1431081565750313TruSeq Adapter, Index 9 (100% over 63bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAT398330.13054014841195433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC129850.057.38772244
TATGCCG133000.055.76896346
GTATGCC136700.054.28481745
ATGCCGT144300.051.44930647
ACGTCTG148050.050.68116815
TCTCGTA144300.050.111341
ACACGTC153100.049.09958613
CGTCTGA153450.049.05480216
TGCCGTC154500.048.09707648
CACACGT156850.047.94769312
GCACACG158700.047.34528411
ATCTCGT157000.045.99186340
CGATCAG171000.043.73943733
TCTGAAC180350.041.9485418
CTGAACT188800.040.19924519
AGTCACG189100.039.77197328
GAGCACA192400.039.321829
TCGGAAG192000.039.2701643
AGCACAC192650.039.252510
AGATCTC186550.038.85445438
Produced by FastQC (version 0.11.2)