FastQCFastQC Report
Tue 8 May 2018
HVG5MBGX5_n01_tga1_rep4_input_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVG5MBGX5_n01_tga1_rep4_input_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32292185
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG104550.039.7616815
GCACACG106300.039.3659911
CACTAGC117200.034.73480231
CGTCTGA120200.034.64212416
ACACGTC124400.033.6384113
CACACGT128900.032.6245912
TATGCCG55700.032.33139446
GTCACTA127800.031.96180529
GTATGCC60500.031.07799145
CGTATGC57150.030.72656644
GAGCACA145350.029.0273729
AGCACAC147200.028.84987310
ACTAGCT144050.028.5239932
GCTTATC146100.027.67548436
AGTCACT150800.027.33898528
CTGAACT156500.026.8936619
AGAGCAC158550.026.6764038
TCGGAAG156450.026.6435813
ATGCCGT61100.026.14264747
TCTGAAC161750.025.99934818
Produced by FastQC (version 0.11.2)